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Tools for handling high-throughput sequencing (genomics) data. Used for reading/writing/editing/indexing/viewing of data in SAM/BAM/CRAM format. More information:

  • Convert a SAM input file to BAM stream and save to file:

samtools view -S -b {{input.sam}} > {{output.bam}}

  • Take input from stdin (-) and print the SAM header and any reads overlapping a specific region to stdout:

{{other_command}} | samtools view -h - chromosome:start-end

  • Sort file and save to BAM (the output format is automatically determined from the output file's extension):

samtools sort {{input}} -o {{output.bam}}

  • Index a sorted BAM file (creates sorted_input.bam.bai):

samtools index {{sorted_input.bam}}

  • Print alignment statistics about a file:

samtools flagstat {{sorted_input}}

  • Count alignments to each index (chromosome/contig):

samtools idxstats {{sorted_indexed_input}}

  • Merge multiple files:

samtools merge {{output}} {{input1 input2 ...}}

  • Split input file according to read groups:

samtools split {{merged_input}}